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The samples were diluted with a phosphate buffer (0.01 M, pH 7.0) to a protein concentration of 1% (w/v). The particle size and polydispersity index (PDI) analyses were performed with a particle size distribution instrument (Nano ZS 90; Malvern Instrument Co., Ltd., Malvern, Worcestershire, UK). The particle size was characterized by the d4,3 (volume average particle size).
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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