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2.2.4. Identification and Calculation of Fatty Acids

BP Beata Paszczyk
MC Marta Czarnowska-Kujawska
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Methyl esters of fatty acids were identified according to their retention times, after comparison with retention times of methyl esters of the reference milk fat fatty acids (BCR Reference Materials) of the CRM 164 symbol, and literature data [51,52,53,54]. The cis9trans11 C18:2 (CLA) isomer was identified using a mixture of CLA methyl esters (Sigma-Aldrich, Germany). To calculate the percentage of fatty acids, the proportions of the peak areas of individual acids in relation to the total area of all identified peaks were used.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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