2.4. Homology and Phylogenetic Analyses of omMc3r

Multiple alignments based on MC3R amino acid sequences of different species were carried out with DNAMAN 9.0 software (Lynnon Biosoft, CA, USA). The calculations of similarity and alignment annotation were made with the software Gendoc (http://www.nrbsc.org/downloads/, accessed on 14 April 2021). The putative transmembrane domains (TMDs) of omMc3r were predicted by the online website NCBI Conserved Domain Search (https://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi?, accessed on 14 April 2021). A phylogenetic tree of amino acids was constructed using MEGA X [24] with the neighbor-joining (NJ) method [25]. Bootstrap values were estimated based on 1000 replicates. GenBank accession numbers of the sequences used in the analyses are presented in a Supplementary Table (Table S1).