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Cell culture

HC Haizhang Chen
AM Andrea Maul‐Pavicic
MH Martin Holzer
MH Magdalena Huber
US Ulrich Salzer
NC Nina Chevalier
RV Reinhard E Voll
HH Hartmut Hengel
PK Philipp Kolb
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All cells were cultured in a 5% CO2 atmosphere at 37°C. BW5147 mouse thymoma cells (BW, kindly provided by Ofer Mandelboim, Hadassah Hospital, Jerusalem, Israel) were maintained at 3 × 105–9 × 105 cells/ml in Roswell Park Memorial Institute medium (RPMI GlutaMAX, Gibco) supplemented with 10% (vol/vol) fetal calf serum (FCS, Biochrom), sodium pyruvate (1×, Gibco) and β‐mercaptoethanol (0.1 mM, Gibco); 293T‐CD20 (kindly provided by Irvin Chen, UCLA; Morizono et al, 2010) were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% (vol/vol) FCS.

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