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Murine wound infection model.

DO David M. P. De Oliveira
BF Brian M. Forde
MP Minh-Duy Phan
BS Bernhard Steiner
BZ Bing Zhang
JZ Johannes Zuegg
IE Ibrahim M. El-deeb
GL Gen Li
NK Nadia Keller
SB Stephan Brouwer
NH Nichaela Harbison-Price
AC Amanda J. Cork
MB Michelle J. Bauer
SA Saleh F. Alquethamy
SB Scott A. Beatson
JR Jason A. Roberts
DP David L. Paterson
AM Alastair G. McEwan
MB Mark A. T. Blaskovich
MS Mark A. Schembri
CM Christopher A. McDevitt
MI Mark von Itzstein
MW Mark J. Walker
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For wound infection, 7-week-old female BALB/c mice were prepared, anesthetized, and subjected to superficial scarification as described previously (21). For infection, A. baumannii MS14413 was cultured to mid-log phase in tryptic soy broth (TSB), and 1 × 106 CFU of bacteria was applied onto the scarified tissue in a final volume of 10 μL. Mice cohorts (n = 10) were treated with combinations of PBT2 (15 mg/kg of body weight; oral gavage), tetracycline (25 mg/kg; intraperitoneal injection), tigecycline (25 mg/kg; intraperitoneal injection), or saline solution (0.9%, wt/vol; intraperitoneal injection and/or oral gavage) in a 100-μL volume. Treatments were administered at 1, 6, 24, and 30 h postinfection. After 2 days of treatment, the mice were euthanized, the skin was excised, and bacteria were enumerated as described previously (19). Statistical differences in CFU were determined by a one-way analysis of variance (ANOVA), with a P value of <0.05 considered statistically significant (GraphPad Prism 9).

Copyright and License information:  ©2022 De Oliveira et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.

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