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Cell viability was detected using CCK-8 kits (CK04; Dojindo Molecular Technologies, Kumamoto, Japan). Neurons at the logarithmic growth phase were seeded in 96-well plates (1 × 104 cells/well) and pre-cultured for 24 h and transfected accordingly. Following the 48-h transfection, neurons were added with 10 μL CCK-8 reagent and incubated at 37 °C for 3 h. Absorbance at 450 nm was detected with a microplate reader.
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