The in vitro cytotoxicity of Fe/CDs@PPSNs against SMMC-7721 cells and NIH-3T3 cells was performed by the MTT assay. In details, SMMC-7721 cells (5000 cells per well) and NIH-3T3 cells (5000 cells per well) were seeded in 96-well plates for 24 h. Then Fe/CDs@PPSNs with different particle concentrations (200, 400, 600, 800, and 1000 ppm) were added to each well and incubated for 24 h. Finally, the cytotoxicity was expressed as the percentage of cell viability as determined by the MTT assay.
Copyright and License information: The Author(s) ©2022 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this
article to respond.