Cell culture

The CT26 murine colon carcinoma cells were cultured in RPMI 1640 supplemented with 10% v/v FBS and 1% v/v penicillin-streptomycin. Murine CD8⁺ T cells were cultured in RPMI 1640 supplemented with 1 mM sodium pyruvate, 1x MEM non-essential amino acids, 1× MEM amino acids, 1 mM l-glutamine, 1× antibiotic-antimycotic, and 0.36 mM 2-mercaptoethanol. Thereafter, these cells were cultured at 37 °C in a humidified air atmosphere containing 5% CO₂. The cell line was authenticated by short tandem repeat (STR) DNA profiling and maintained in low passage (< 15) for the present study.