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Plant materials and treatment

PV P. Vignesh
CM C. Mahadevaiah
RP R. Parimalan
RV R. Valarmathi
SD S. Dharshini
SN Singh Nisha
GS G. S. Suresha
SS S. Swathi
HS H. K. Mahadeva Swamy
VS V. Sreenivasa
KM K. Mohanraj
GH G Hemaprabha
RB Ram Bakshi
CA C. Appunu
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Salinity-tolerant Erianthus arundinaceus genotype IND99-907 collected from the saline lands from Ernakulum, India29 and salinity-sensitive sugarcane genotype Co 9701030,31 were used in this study. Single buds from each genotype with five replications were grown in pots containing river sand: red soil: farmyard manure in 1:1:1 proportion. The experiment was conducted in the polyhouse facility with a 16-h photoperiod, 30/25 °C day/night, 380 − 400 μmolm−2 s−1. Salt stress was imposed during the formative stage on the 90th day after planting by irrigating with salinized water containing NaCl, CaCl2, and Na2SO4 in the ratio of 2:2:130. Salinity stress was imposed by adding 25 mM of saline water on the first day followed by incrementally adding 25 mM on every day till reaches 175 mM100.

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