2.2. Genome sequencing, assembling and annotation

Sequencing libraries were generated by a TruSeq Nano DNA LT Kit (Illumina, San Diego, CA). The final libraries were run on an Agilent 2100 Bioanalyzer to verify the fragment size distribution and concentration. Sequencing was performed at Genoma Mayor (Universidad Mayor, Chile) with an Illumina sequencing platform. Paired-end sequences of 150 bp were generated for each read (R1 and R2). The filtered reads were assembled using SPAdes 4 software version 3.13.0 (Bankevich et al., 2012), using three k-mers parameters: - k 33, 55 and 77. The chloroplast was annotated with DOGMA software (Wyman et al., 2004) and CPGAVAS2 (Shi et al., 2019), and then manually corrected. The graphical map of the chloroplast was generated by Organellar Genome DRAW (OGDRAW) (Greiner et al., 2019), and the complete nucleotide sequence was deposited in the GenBank database ({"type":"entrez-nucleotide","attrs":{"text":"MW348956.1","term_id":"1963749270","term_text":"MW348956.1"}}MW348956.1, under the name R. phycelloides)