Assessment of DSAs and Calculated PRA by Luminex Technology

Anti-HLA antibodies were assessed before transplantation and during the per-protocol and the for-cause renal biopsy through Luminex-based technology (22). Briefly, antibodies were tested using Single Antigen Bead test (LIFECODES® Single Antigen, Immucor, Georgia, USA). An allele was considered positive if the MFI was over 1,500 and was x4 times higher than the Lowest Reactive Antigen (LRA) of the same locus. Calculated PRA (cPRA) was determined using a panel of 500 unrelated individuals from local population and was typed for A, B, C, DRB1, and DQB1 in high resolution (2 fields) by sequence-based typing.