Cell Electroporation with SGM, SAM, and pmaxGFP Plasmid

Cells were harvested by trypsinization, and 2 × 10⁶ cells were pelleted by centrifugation at 100g for 3 min. Cells were resuspended in Nucleofector solution from Lonza. SF cell line 4d-Nucleofector X kit S (V4XC-2032) for HepG2 cells. Cells were electroporated with 0.4 μg of pmaxGFP plasmid and different concentrations of SGM and SAM (0.01, 0.1, 1 mM) using 4D-Nucleofector X Unit from Lonza. EH-100 program was used for HepG2 by following the manufactures protocol. Cells were incubated overnight in the incubator and observed under the fluorescence microscope. Cells were observed using Leica DMiL microscope using 10× objective. For higher magnification, cells were observed using a ZEISS Celldiscoverer 7 using a 20× objective with a 2× magnification changer.