Antigen-induced Pulmonary Inflammation (AIPI)

Female B10Q, B10Q.Ncf1 ^* and B10Q.Ncf1 ^*.MN mice were used to induce AIPI model essentially following previously described protocols (10). A colloid emulsion was prepared by mixing 0.1 mg/ml OVA (Sigma) and Imject™ Alum (Pierce, Thermo Fisher Scientific) in equal volume. The mice were sensitized by intraperitoneal injection of the OVA emulsion (200 μl per mouse) at days 0 and 7. From day 14, the mice were challenged with 1% OVA aerosol for 30 min per day for 7 consecutive days. On day 21, blood, bronchoalveolar lavage fluid (BALF) and lung tissues were collected.