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Photosensitization and light source

FA Farheen Akhtar
AK Asad U. Khan
BQ Bushra Qazi
SK Senthilguru Kulanthaivel
PM Prashant Mishra
KA Kafil Akhtar
AA Asif Ali
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Red diode laser (Model No-MRL-III-630; CNI, China) was used for photosensitization.

The effective radiant exposure of the light source was calculated using the following formula66:

where power density (PD) = P (mW)/Area (cm2), P represent the output power (100 mW) of laser and A indicates the area of irradiation. In this study, the laser treatment was carried out in a U-bottom microtiter plate. Thus, the samples acquire the shape of a hemisphere; hence the irradiated area was 2πr2, where r is the radius of the laser beam exposed, which is equal to 0.35 cm. The beam height from the base was 24.8 mm. Thus, the value of applied PD was 0.1300 W/cm2 and the energy fluency was set to 100 J/cm2 (12 min and 50 s) based on above mentioned formula.

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