2.6.1. T Cell Sorting

The tumor tissues were ground and filtered to obtain cell suspensions. The cells were collected by centrifugation at 1000 RPM for 5 min and washed with PBS three times, and the supernatant was removed by centrifugation. Then cells were fixed via a cell fixation solution for 30 min and permeabilized by 1% Triton-100 at 37 °C for 10 min, followed by antibody incubation (CD3-PE: 0.25 μg/Test; CD4-FITC: 0.25 μg/Test; CD8a-FITC: 1 μg/Test; CD25-Cy7: 0.25 μg/Test; Foxp3-APC: 1 μg/Test, BioLegend, San Diego, CA, USA) at 37 °C in the dark for 30 min. The cell phenotypes were detected by flow cytometry after 500 μL PBS was added.