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4.4. Measurement of Blood Metabolites and Glycogen in Hearts

YU Yogi Umbarawan
RK Ryo Kawakami
MS Mas Rizky A. A. Syamsunarno
HO Hideru Obinata
AY Aiko Yamaguchi
HH Hirofumi Hanaoka
TH Takako Hishiki
NH Noriyo Hayakawa
NK Norimichi Koitabashi
HS Hiroaki Sunaga
HM Hiroki Matsui
MK Masahiko Kurabayashi
TI Tatsuya Iso
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Serum levels of glucose (glutest sensor; Sanwa Kagaku, Aichi, Japan), triglyceride (Triglyceride E-test; Wako Chemical, Osaka, Japan), non-esterified fatty acid (NEFA C-test; Wako Chemical, Osaka, Japan), β-hydroxybutyrate (EnzyChrom Ketone Body Assay Kit; BioAssay Systems, Milpitas, CA, USA), insulin (Mouse Insulin ELISA; Mercodia, Uppsala, Sweden) and lactate (Lactate Colorimetric Assay Kit; BioVision, Milpitas, CA, USA) were measured according to the manufacturers’ protocols [9,14,26]. Glycogen in hearts was measured as described previously [30].

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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