2.4. Gene Duplication and Synonymous and Non-Synonymous Substitution Rate

CW Cuicui Wu
DZ Dongyun Zuo
SX Shuiping Xiao
QW Qiaolian Wang
HC Hailiang Cheng
LL Limin Lv
YZ Youping Zhang
PL Pengbo Li
GS Guoli Song
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Gene duplication in cotton species was executed by DupGen_finder (https://github.com/qiao-xin/DupGen_finder, accessed on 10 July 2021). Selection pressure analysis was performed by calculation of Ka (non-synonymous substitution rate) and Ks (synonymous substitution rate) values of homologous genes using KaKs_Calculator 2.0 software [38], with NG methods to calculate the Ks/Ka ratios of homologous gene pairs.

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