2.3. Swarming Inhibition

Swarming inhibition was determined as previously reported [54]. Three milliliters of M9 (0.6% agar) was added per well with the different treatments in 6-well plates (Corning^®, Corning, NY, USA) and allowed to dry under sterile conditions for 45 min. Subsequently, 25 μL of an overnight culture of P. aeruginosa was adjusted to an O.D._{600 nm} = 0.08 and inoculated in the center of each well. The plates were incubated at 37 °C for 24 h in a humid chamber (Thermo Scientific^®, Walthan, MA, USA), and the ImageJ^® program (Bethesda, MD, USA) was used to calculate the swarming area.