3.6. Determination of Minimum Inhibitory Concentration (MIC)

The antibacterial potential of F-AgNPs was calculated by their MICs against Staphylococcus aureus (MDR), Klebsiella pneumonia, Salmonella abony, and Escherichia coli (MDR). The bacterial strain was collected in the mid-logarithm phase and diluted to 2 × 10⁵ CFU/mL in 0.03% of Luria-Bertani (LB) broth in PBS. The 100 μL of LB medium consist of F-AgNPs were serially diluted in 96 well plates [22]. The lowest concentration of F-AgNPs at which 25, 50, 75% growth of microbes was inhibited is defined as MIC₂₅, MIC_50, and MIC₇₅, respectively. For negative control, Milli-Q water was used for each experiment.