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Protein concentrations in the urine samples were determined according to the manual of the Rat Urinary Protein Assay Kits used here (Chondrex, Woodinville, WA, USA). Urine samples were centrifuged at 10,000 rpm for three minutes. 3% sulfosalicylic acid was added to each sample and 0.1 N HCl was added as a blank. The samples were incubated for ten minutes at room temperature and read using a plate reader at OD 450 nm.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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