2.5. Kaplan–Meier Survival Curves

To determine whether rHJ mAb was effective as a candidate treatment for sepsis, groups of mice were injected IV with LPS at 6.0 mg/kg (an LD₈₀ dose of LPS) and 0, 2, and 6 h later were treated with either saline (control) or low-dose rHJ mAb (125 ng/gm body weight) or high-dose rHJ mAb (1.25 µg/gm body weight). The number of mice surviving was checked daily for 7 days, and the results were plotted as Kaplan–Meier survival curves. Two additional mouse models of sepsis were also utilized: one used an LD₈₀ IV dose of TNFα, and the other used an LD₈₀ IV dose of MV-A iNOS [8,33].