Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

2.2. Sample Collection

GM George Michail
LK Lefkothea Karapetsi
PM Panagiotis Madesis
AR Angeliki Reizopoulou
IV Ioannis Vagelas
request Request a Protocol
ask Ask a question
Favorite

For this study, we used subsamples from a stalactite collected using gloves in a plastic sterile container that is symmetrically shaped and has a diameter of 7.5–8 cm. The stalactite sample was collected in 2021 and was more than 50 m deep inside the Agios Athanasios cave (Figure 1). In this paper, we present taxonomic results obtained by metataxonomics (16S rRNA gene sequencing) from only one stalactite sample and not more, as there are no statistically significant changes obtained among phyla, families, and genera, as presented by Durazzi et al. [23].

Formation of Stalactites inside cave Agios Athanasios, Central Thessaly, Greece.

Copyright and License information:  ©2021 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A