request Request a Protocol
ask Ask a question
Favorite

Burkholderia cepacia selective agar (BCSA; product ref: 33631) was obtained from bioMérieux, Craponne, France, as pre-poured plates. BD Cepacia medium (product ref: 256180) was obtained from Becton-Dickinson, Rungis, France as pre-poured plates. MAST Cepacia medium was prepared weekly in our laboratory using materials purchased from MAST Diagnostics Ltd., Bootle, UK. Preparation was performed in exact accordance with manufacturer’s instructions using dehydrated B. cepacia medium (Product ref: DM253D;) supplemented at 45 °C with B. cepacia Selectavial (Product code: SV-22). MAST cepacia medium was used within 7 days of preparation and other media were used before their specified expiry date. Columbia blood agar (Oxoid, Basingstoke, UK) was used for general subcultures.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A