4.1. Instruments and Experimental Equipment

Costar-clear 96-well culture plates were purchased from Costar (New York, NY, USA). Wellwash 4MK2 automatic plate washer (Thermo, Waltham, MA, USA) and SpectraMax M2e phosphase marker were from Molecular Instruments (Molecules, Los Angeles, CA, USA). Ultrasonic Cleaner (Jiangsu KH-500E) was from Kunshan Hehuang Ultrasonic Instrument Co., Ltd. (Kunshan, China). Shimadzu Prominence LC-20AT high performance liquid chromatography system (Kyoto, Japan) consisted of two LC-20AT solvent delivery units, one SIL-20A autosampler, one SPD-M20A photodiode array detector, one CBM-20Alite systemcontroller, and one Synergi reversed-phase Hydro-C18 column (250 mm, 4.6 mm, 10 mm) (Phenomenex, Torrance, CA, USA).