4.7. DNA Transfection for nc886 Overexpression and siRNA Transfection for nc886 Knockdown

To obtain DNA for overexpression of nc886 in cells, DNA was amplified with Accu-Power^® PCR PreMix (BIONEER, Daejeon, Korea) using genomic DNA of HDFs as template and the following primers described in Table 2.

Sequences of primers used DNA Transfection for nc886.

Amplified DNA was purified using a QIAquick PCR Purification Kit (QIAGEN, Hilden, Germany). The purified DNA was transfected with a LipofectamineTM 3000 reagent (Thermo Fisher Scientific, Waltham, MA, USA). To knockdown nc886, cells were transfected with anti-oligos (si-ctrl and si-nc886) at a concentration of 250pM using the Lipofectamine™ RNAiMAX reagent (Thermo Fisher Scientific, Waltham, MA, USA).