2.2. Preparation of Antibiotics Loaded Core Biomaterial Inks

Respective antibiotic powders (Vancomycin HCl, Clindamycin HCl and Gentamicin sulphate, all provided by Heraeus Medical, Germany) were first dissolved in ddH₂O at a concentration of 20 mg/mL. Additionally, 6% (w/v) alginate sol in ddH₂O was prepared. Antibiotic solutions were added drop wise to the stirring 6% alginate solution. An equal volume of both the solutions was used, so that the final concentration of the antibiotics and alginate in the resultant suspension was 10 mg/mL and 3% (w/v), respectively. The antibiotic loaded alginate solution was used as a core part to fabricate 3D core/shell scaffolds (Section 2.4).