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Quantification: pericyte coverage ratio and density

DK Deepak Kumar Kaushik
AB Anindita Bhattacharya
BL Brian Mark Lozinski
VY V. Wee Yong
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The pericyte coverage ratio along the blood vasculature in cerebellar sections was quantified at three time periods in the EAE model (day 10–13, day 16, and day 21) and in naïve animals. Confocal images from cerebellar tissues stained to visualize pericytes, using the markers NG2 and PDGFRβ, and blood vessels through CD31, were assessed. Three naïve and three EAE animals were examined at each time point and three fields of view were quantified per animal. The pericyte coverage ratio was quantified by measuring the total length of the blood vessels in each field of view and the total length of the blood vessel covered by pericytes in each field of view. The pericyte coverage ratio in 60× confocal images having an area of 215 μm × 215 μm was calculated as follows:

To quantify pericyte density, the number of pericytes along the blood vasculature was quantified in each 60× confocal image, having an area of 215 μm × 215 μm.

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Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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