Fetal bovine serum (FBS) and culture media were obtained from Invitrogen (Grand Island, NY, USA). Bovine serum albumin (BSA), crystal violet and bacterial lipopolysaccharide (LPS) were purchased from Sigma-Aldrich (St Louis, MO, USA). Bradford reagent, acrylamide, immunoblot PVDF membranes and chemiluminiscent HRP Substrate were purchased from Bio-Rad (Madrid, Spain).
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