Secretory IgA concentrations were determined to evaluate the local immunity status in different disease conditions, including respiratory diseases such as pneumococcal disease. The two-site sandwich enzyme immunoassay for secretory IgA was purchased from Bio Vendor Research and Diagnostic Products (Brno, Czech Republic), with a sensitivity assessed as 0.6 µg/mL. The lower detection limit was 57 µg/mL for saliva samples, and the upper limit was fixed at 260 µL/mL.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.