Male CD-1 mice, 25 to 30 g in weight and 5 to 6 weeks of age (Harlan Iberica, Barcelona, Spain), were housed in standard polypropylene cages with ad libitum access to food and water inside a controlled environment room kept at 22 °C ± 1 °C with a 12 h light and 12 h dark cycle at the Faculty of Pharmacy Central Animal Facility, University of Lisbon, Lisbon, Portugal.
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