2.8.1. Genomic DNA Extraction and Amplicon Generation

Total genome DNA was extracted from samples with the Cetyltrimethylammonium Bromide and sodium dodecyl sulfate (CTAB/SDS) method. DNA concentration and purity were monitored on 1% agarose gels. According to the monitored concentration, DNA was diluted to 1 ng/μL with sterile water. The 16S rRNA genes of distinct regions were amplified by using specific primer with the barcode. All PCR reactions were carried out with Phusion® High-Fidelity PCR Master Mix with GC Buffer (New England Biolabs, Beverly, MA, USA).