Each accession was germinated under controlled environmental conditions (Fitotron, Weiss-Technik UK, Loughborough, UK). Long-day lighting was used (16 h light, 8 h dark) at an intensity of 200 μmol m−2 s−1. Day temperatures were set at 20 °C and night temperatures at 14 °C. Seedlings were grown for 10 days in seedling trays and then transplanted to larger trays. Subsequently, plants were grown for a further 20 days and then leaves were harvested at 30 days. Leaves were collected in batches of 70 g in three experimental replicates per accession. Amount of leaf material was chosen based on preliminary experiments, where abundant yields of VOCs were obtained.
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