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The cells were seeded into 96-well plates (1000 cells/well) and cultured for 12h, 24h, 48h, and 72h. The viability of the cells was assessed using a CellTiter-Blue® (CTB) cell viability assay (CTB169, Promega, Beijing, China). At varying points in time, 20 μl of [3‐(4,5‐dimethylthiazol‐2‐yl) ‐5‐ (3-carboxymethoxyphenyl)-2‐(4-sulfophenyl)-2H-tetrazolium (MTS) reagent was added to each well, and incubated for 2 h at 37°C. The absorbance was measured at a wavelength of 490 nm to detect the OD values. All experiments were performed in triplicate.
Copyright and License information: ©2021 Lv, Cui, Huang, Feng, Wang, Wang, Xuan, Li, Ma, Huang and Zhang
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