Plasma was collected from mice after infection and kept at − 80 °C. Antibodies used in ELISA were purchased from eBioscience, Biolegend or BD Pharmingen including standard protein, purified, and biotinylated antibodies. Paired antibodies were used to perform ELISA for corresponding cytokines per the manufacturer’s recommendations.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.