Cytokine detection by ELISA

ML Mingyue Li
MB Mayassa J. Bou-Dargham
JY Jiyeon Yu
ZE Zienab Etwebi
HS Honghong Sun
YC Youhai H. Chen
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Plasma was collected from mice after infection and kept at − 80 °C. Antibodies used in ELISA were purchased from eBioscience, Biolegend or BD Pharmingen including standard protein, purified, and biotinylated antibodies. Paired antibodies were used to perform ELISA for corresponding cytokines per the manufacturer’s recommendations.

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