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1,9-Dimethylmethylene blue (DMMB) colorimetric assay for sulfated glycosaminoglycans

MY Matthew J. Yousefzadeh
RF Rafael R. Flores
YZ Yi Zhu
ZS Zoe C. Schmiechen
RB Robert W. Brooks
CT Christy E. Trussoni
YC Yuxiang Cui
LA Luise Angelini
KL Kyoo-A Lee
SM Sara J. McGowan
AB Adam L. Burrack
DW Dong Wang
QD Qing Dong
AL Aiping Lu
TS Tokio Sano
RO Ryan D. O’Kelly
CM Collin A. McGuckian
JK Jonathan I. Kato
MB Michael P. Bank
EW Erin A. Wade
SP Smitha P. S. Pillai
JK Jenna Klug
WL Warren C. Ladiges
CB Christin E. Burd
SL Sara E. Lewis
NL Nicholas F. LaRusso
NV Nam V. Vo
YW Yinsheng Wang
EK Eric E. Kelley
JH Johnny Huard
IS Ingunn M. Stromnes
PR Paul D. Robbins
LN Laura J. Niedernhofer
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For each mouse, nucleus pulposus tissue isolated from four lumbar IVDs of each mouse were pooled and digested using papain at 60 °C for 2 h. GAG content was measured in duplicates by the DMMB procedure using chondroitin-6-sulfate (Millipore Sigma C-8529) as a standard41. The DNA concentration of each sample was measured using the Pico-Green assay (Molecular Probes) and used to normalize the glycosaminoglycan values.

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