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At the end of the feeding period, animals were anesthetized with 10% chloral hydrate (350 mg/kg body weight). Blood was collected using cardiac puncture into tubes containing 0.1% EDTA following an overnight fast. Blood was then centrifuged at 5,500 × g for 12 min at 4°C to separate the plasma. The plasma was immediately used to determine the levels of LDL-C, high-density lipoprotein cholesterol (HDL-C), total cholesterol (TC), and triglyceride (TG) using an automatic biochemistry analyzer (AU480 Chemistry system; Beckman Coulter, Inc., Brea, CA, USA).
Copyright and License information: ©2016 Zhao et al.
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
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