Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Antibody

XS Xikui Sun
JB Jingning Bai
JX Jiejie Xu
XX Xiaoli Xi
MG Mingyu Gu
CZ Chengming Zhu
HX Hongman Xue
CC Chun Chen
JD Junchao Dong
request Request a Protocol
ask Ask a question
Favorite

The primary antibodies used in this study were as follows: anti-ATM Rabbit antibody (D2E2, #2873, Cell Signaling Technology), anti-γ-Tubulin antibody (#5886, Cell Signaling Technology), anti-Mre11 Antibody (#4895, Cell Signaling Technology), anti-DNA-PKcs (G-12, SC-390849, Santa Cruz), anti-CtIP (D-4, SC-271339, Santa Cruz), anti-EXD2 antibody (20138-1-AP, Proteintech), anti-phospho KAP1 (S824) antibody (A304-146A-M, Bethyl Laboratories), anti-β-Actin antibody (66009-1-Ig, Proteintech), anti-AID monoclonal antibody (mAID-2, 14-5959-82, eBioscience), and anti-Flag M2 antibody (F1804-50UG, Sigma-Aldrich). The antibodies for flow cytometry analysis were anti-Mouse lgM-APC (17-5790-82, eBioscience), anti-Mouse lgA-PE (12-4204-83, eBioscience), and anti-Mouse lgG1-PE (406608, Biolegend).

Copyright and License information:  ©2021 Sun, Bai, Xu, Xi, Gu, Zhu, Xue, Chen and Dong.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A