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The frozen cells were thawed in the 37°C water bath, centrifuged (300 g, 7 min) to remove the remaining cryoprotectant and suspended in the MACS medium. After 30 min of incubation at 37°C to restore their membranes the cells’ viability was estimated using the Trypan Blue test and the Nikon Eclipse E 200 (Nikon) light microscope at ×20 magnification. Using hemocytometer chamber all living and dead cells are counted and the alive are presented as a percentage of all cells.
Copyright and License information: ©2021 Semenova, Grudniak, Bocian, Chroscinska-Krawczyk, Trochonowicz, Stepaniec, Murzyn, Szablowska-Gadomska, Boruczkowski, Oldak and Machaj.
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