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scRNA-seq with BD Rhapsody platform

HZ Honghui Zeng
LW Le Wang
JL Jiajia Li
SL Siweier Luo
QH Qianqian Han
FS Fang Su
JW Jing Wei
XW Xiaona Wei
JW Jianping Wu
BL Bin Li
JH Jingang Huang
PT Patrick Tang
CC Chunwei Cao
YZ Yiming Zhou
QY Qiongqiong Yang
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Sorted peripheral blood mononuclear cells were resuspended in PBS with 10% FBS and then centrifuged at 1000 r.p.m. for 5 min and resuspend in 200 μL staining buffer (BD Biosciences, San Jose, USA). Cells from each person were incubated with 10 μL one BD SMK sample tag (633781, BD Biosciences, San Jose, USA) for 20 min at RT and washed with cold-PBS twice. After the sample tag labeling, PBMCs from four people (at least one from healthy donors) were mixed for one scRNA-seq experiment. The average cell number of scRNA-seq experiments was around 15,000 cells. The read depth of each experiment was sequenced to 100 K reads per cell. Single-cell mRNA capture and library construction were performed according to BD Rhapsody's official instructions. Next-generation sequencing was performed using the Illumina Nova-Seq platform according to the official instructions.

Copyright and License information: The Author(s) ©2021
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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