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RNA extraction

KV Kelly Voo
JC Jeralyn Wen Hui Ching
JL Joseph Wee Hao Lim
SC Seow Neng Chan
AN Amanda Yunn Ee Ng
JH Jasmine Yi Ying Heng
SL Shiao See Lim
JP Jun Wei Pek
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RNA extraction was performed as described previously [15], using TRIzol (Ambion) and the Direct-zol miniprep kit (Zymo Research). To detect maternally deposited sisRNA and mRNA, stage 14 oocytes were manually isolated to avoid contamination with pre-mRNA in transcriptionally active germline cells. For experiments involving detection of pre-mRNA and mRNA for gene expression and splicing efficiency, whole ovaries were used as they reflect the steady-state levels of pre-mRNA and mRNA as described in previous studies [32,33].

Copyright and License information:  ©2021 Voo et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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