C-FOS immunoreactivity: marker of iN activity

CL Célia Lentini
Md Marie d’Orange
NM Nicolás Marichal
MT Marie-Madeleine Trottmann
RV Rory Vignoles
LF Louis Foucault
CV Charlotte Verrier
CM Céline Massera
OR Olivier Raineteau
KC Karl-Klaus Conzelmann
SR Sylvie Rival-Gervier
AD Antoine Depaulis
BB Benedikt Berninger
CH Christophe Heinrich
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To evaluate whether iNs were physiologically active, we counted for each mouse the number of DSRED/NEUN+ iNs exhibiting C-FOS+ nuclei that was expressed as percentage of the total number of DSRED/NEUN+ iNs. For each group of mice (in situ iNs, n = 3, Figure 5G; graft-derived iNs, n = 3 without CNO, n = 4 after CNO treatment, Figure 6C), values are given as mean percentages ± SEM.

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