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4.6. Primary HUVEC Cell Culture

EG Eveline A. C. Goossens
LZ Licheng Zhang
MV Margreet R. de Vries
JJ J. Wouter Jukema
PQ Paul H. A. Quax
AN A. Yaël Nossent
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HUVECs were cultured at 37 °C in a humidified 5% CO2 environment with HUVEC culture medium (EBM-2 Basal Medium (CC-3156) and EGMTM-2 SingleQuotsTM Supplements (CC-4176), Lonza). Media were refreshed every 2–3 days. Cells were passed using trypsin (Sigma) at 70–80% confluency. HUVECs were used for the scratch-wound healing assay at passage three. HUVECs were stored up to passage three in 90% heat inactivated New Born Calf Serum (NBSCi) (Sigma) and 10% DMSO (Sigma).

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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