4.9. Golgi–Cox Staining

A rodent anesthetic vaporizer (VetFloTM, Kent Scientific Corporation, Torrington, CT, USA) was used to anesthetize the mice using isoflurane. Brains were promptly taken from the skulls of anesthetized mice, washed with 0.1 M PBS (+4 °C), and sliced into two hemispheres. According to the manufacturer’s instructions, the material was stained with the FD Rapid GolgiStain^TM kit (FD NeuroTechnologies, Ellicott City, MD, USA). A cryomicrotome was used to make 100-m thick slices (HM 550; Thermo Scientific, Waltham, MA, USA). Slices were mounted on gelatin-coated slides, stained, dehydrated, and coverslipped with VectaMount^TM mounting medium (H-5000; Vector Laboratories, Burlingame, CA, USA).