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HeLa cells were seeded at a density of 100000/ml in 24-well plates on coverslips. The following day cells were treated with either 0 nM, 25 nM or 250 nM Apilimod, 100 nM or 1 μM YM201636 or 10 mM ammonium sulfate for 4 h. One micromolar lysotracker Red was added to cells for 5 min, followed by fixation with 4% formaldehyde in PBS, washed twice with PBS and mounted using Mowiol.
Copyright and License information: ©2016 The Author(s)
This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution Licence 4.0 (CC BY).
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