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Study design and sample collection

WM Willem R. Miellet
RM Rob Mariman
GP Gerlinde Pluister
LJ Lieke J. de Jong
IG Ivo Grift
SW Stijn Wijkstra
EL Elske M. van Logchem
JV Janieke van Veldhuizen
MI Marie-Monique Immink
AW Alienke J. Wijmenga-Monsuur
NR Nynke Y. Rots
ES Elisabeth A. M. Sanders
TB Thijs Bosch
KT Krzysztof Trzciński
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In the fall of 2018, saliva and oropharyngeal swabs were collected from college students of Hogeschool Utrecht (n = 300). After signing informed consent, students self-collected saliva by spitting 1 ml into a 15 ml tube (Greiner, Kremsmünster, Austria). Next, a study nurse swabbed student’s posterior pharyngeal wall with a nylon swab (FLOQSwabs, COPAN, Brescia, Italy) to collect an oropharyngeal sample. Immediately after collection, saliva (approximately 50 µl) and oropharyngeal swab were used to inoculate Neisseria Selective Medium PLUS agar plates (NS-agar, Oxoid, Badhoevedorp, the Netherlands) and within 20 min plates were placed in a 37 °C, 5% CO2 incubator. Once all samples have been collected, cultured plates were transported at room temperature to the laboratory.

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