2.3. Instrumentation and chemicals

UV spectra scanned in methanol on a Lambda Bio 20 spectrophotometer (Shimadzu-U, Singapore). IR spectra were recorded in KBr pellets on a Win IR FTS 135 instrument (Biorad, Philadelphia, PA, USA). ¹H nuclear magnetic resonance (NMR) (300 MHz) and ¹³C NMR (75 MHz) spectra were recorded on a Brucker spectrometer (Brucker, Billerica, MA, USA) in Deuterated methanol (MeOD) with Tetramethyl silane (TMS) as internal standard. The Mass Spectroscopy were measured on a JEOL-AccuTOF machine, Ambala, Haryana, India equipped with a Direct Analysis in Real Time (DART) ion source and helium was used as a gas of collision. Melting points were determined on Perfit melting point apparatus, Ambala, Haryana, India. Silica gel (60–120 mesh, Qualigens, Mumbai, India) was used for column chromatography. Silica gel G (Qualigens) was used for analytical thin layer chromatography. Spots were visualized by exposure to iodine vapors, a UV lamp (254 nm), and by spraying with anisaldehyde sulfuric acid reagents. 1,1-Diphenyl-2-picrylhydrazyl (DPPH, Sigma-Aldrich, Bangalore, Karnataka, India). The solvents for isolation were obtained from Merck Mumbai, India.