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Tumors and tissues were fixed in 4% neutral buffered formalin solution for more than 24 h and embedded in paraffin. Sections 3–5 μm of the tissues were used to measure the effect of MIL on inducing apoptosis using TUNEL assay (In Situ Cell Death Detection Kit, Roche, Mannheim, Germany). The effect of MIL on NF-κB (p65) would be measured using NF-κB (p65) (Mouse, Santa Cruz, sc8008) staining.
Copyright and License information: ©2016 Wu et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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