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Colony formation and radiosensitivity assays
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HNE1 cells were seeded at 400 cells/well in six-well plates in triplicate and divided into 2 groups: radiotherapy group and combined 4EGI-1 treatment group. After a 24h rest, 4EGI-1 was applied in 0μM and 12.5μM according to the corresponding groups. Twenty-four hours later, after replacing the fresh complete medium, cells were treated once with radiation (0, 2, 4, 6 or 8 Gy). The colony formation assay was conducted as previously described [40].
Copyright and License information: ©2016 Wang et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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