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The 3D printed structures of microbial ink (CsgA-αγ) embedded with PQN4-BPA biofilm were immersed in water with or without 1 mM BPA and incubated for 24 h. After 12 and 24 h the water spiked with BPA was collected to test BPA binding by the microbial ink embedded with PQN4-BPA biofilm. The presence of BPA was detected using Agilent 6460 Triple Quad LC/MS and Agilent 1290 Infinity HPLC by Small Molecule Mass Spectrometry Facility at Harvard University, MA, USA. Data obtained from at least three independent samples were reported.
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