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Sodium dodecyl sulphate was added to a final concentration of 0.015% to each polysome fraction and incubated for 30 minutes in room temperature. Tri-chloroacetic acid (TCA) was added to polysome fractions at 25% of their volume. All the fractions were incubated for 30 minutes post-TCA addition followed by centrifugation at 13,000g for 30 minutes at room temperature. The pellets were washed with ice-cold acetone (Merck) twice and dried. Acetone residues were allowed to evaporate and the pellets were resuspended in Laemmli buffer.
Copyright and License information: ©2021 Srinivasan et al ©2021 Lucas SmithThis is an open access article distributed under the terms of the , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ©2021 Lucas SmithThis is an open access article distributed under the terms of the , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ©2021 Lucas SmithThis is an open access article distributed under the terms of the , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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